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US 9,989,526 B2Granted
Method and device for bioassays
AssigneeAbionic SA
InventorsNicolas Durand, Iwan Märki, Annick Mayor, Stéphane Broillet
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Abstract
A method and device for multiplexing and calibrating rapid quantification of biomolecules present in a nanofluidic biosensor composed by a nanoslit. A novel concept defining multiple different local structured areas containing biomarkers. Local structured areas can also be structured to decrease the biomarkers density in the nanoslit. Such enables the multiplexed quantification biomolecular interactions of interest in the same nanofluidic biosensor.
Claims15
A biosensor for detecting and quantifying fluorescently-labeled biomolecules, said biosensor comprising:
- a lower substrate;
- an upper substrate stacked on said lower substrate;
- a nanoslit which is a microfabricated structure with one-nanometer-sized dimension, formed between said lower substrate and said upper substrate, said nanoslit comprising a plurality of local structured areas disposed along said nanoslit;
- a lateral input aperture in direct contact with said nanoslit, for letting a solution containing biomolecules enter said nanoslit;
- and
- a lateral output aperture in direct contact with said nanoslit, for driving said solution through said nanoslit, wherein the plurality of local structured areas are each functionalized by different biomarkers.
3 more elements
Dependencies are inferred from the claim text.