Generation of airway and lung progenitors and epithelial cells and three-dimensional anterior foregut spheres
AssigneeThe Trustees of Columbia University in the City of New York
InventorsHans-Willem Snoeck, Sarah Xuelian Huang, Ya-Wen Chen, Jahar Bhattacharya, Mohammad Naeem Islam
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Abstract
Embodiments of the present invention are directed to various methods for generating airway and lung progenitors and epithelial cells and three-dimensional anterior foregut spheres, and to populations of cells made using the methods. The airway and lung progenitors and epithelial cells can be used as a model to study diseases that primarily affect airway epithelial cells, and to study human lung development. Methods are also provided for drug screening. Anterior foregut spheres can be used as a model for lung fibrosis.
Claims15
A method comprising culturing-Anterior Foregut Epithelial-(AFE) cells in a Wnt signaling agonist, retinoic acid (RA) at a concentration of from 10 nanomoles to 100 nanomoles, 10 to 50 nanomoles, or 50 to 100 nanomoles and an agent that activates BMP4 signaling or BMP4 for at least 9 days, thereby producing lung and airway progenitor cells that express both NKX2.1 and FOXA2.
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