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US 9,987,307 B2Granted
Tolerogenic dendritic cells, methods of producing the same, and uses thereof
AssigneeSOTIO a.s.
InventorsLenka Palová Jelínková, Klára Dáñová, Radek S̆pí{hacek over (s)}ek
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Abstract
Described herein are methods for the preparation of stable semi-mature tolerogenic dendritic cells and compositions comprising such stable semi-mature tolerogenic dendritic cells. The stable semi-mature tolerogenic dendritic cells described herein and compositions thereof can be used for the establishment of immune tolerance when treating an autoimmune disease, graft rejection and/or graft-versus-host disease.
Claims25
A method for generating stable semi-mature tolerogenic dendritic cells, the method comprising:
- a. culturing monocytes isolated from a subject's blood in culture medium comprising GM-CSF and IL-4;
- b. after a first period of time in culture, culturing the cells from step (a) in culture medium comprising Dexamethasone, GM-CSF and IL-4;
- c. after a second period of time in culture, culturing the cells from step (b) in culture medium comprising Dexamethasone, vitamin D2, GM-CSF and IL-4 for a third period of time to generate tolerogenic dendritic cells;
- and
- d. after third period of time in culture, culturing the tolerogenic dendritic cells in culture medium comprising MPLA, GM-CSF and IL-4, or MPLA, GM-CSF, IL-4 and an antigen(s) associated with an autoimmune disease, graft rejection or graft-versus-host disease;
- wherein the cells from step (a) are cultured in culture medium comprising Dexamethasone, GM-CSF and IL-4 on the 3rd day in culture, and
- wherein the cells from step (b) are cultured in culture medium comprising Dexamethasone, vitamin D2, GM-CSF and IL-4 on the 6th day of culture, and
- wherein the tolerogenic dendritic cells are cultured in culture medium comprising MPLA, GM-CSF and IL-4, or GM-CSF, IL-4, MPLA and the antigen(s) on the 7th day of culture.
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