US 9,982,269 B2 · Mortierella alpine uracil auxotroph with URA5 gene knocked out through homologous recombination, and construction method thereof

Abstract

It relates to a Mortierella alpine ATCC32222 uracil auxotroph strain and a construction method thereof. In the present invention, Mortierella alpine ATCC32222 is used as a material and undergoes gene knockout through an Agrobacterium tumefaciens mediated genetic manipulation technology, to obtain the Mortierella alpine uracil auxotroph. The method is of great significance for the basic theoretic researches of the oil producing fungus Mortierella alpine ATCC32222 and product development.

Claims1

Dependent claims

An isolated Mortierella alpina MAU1 deposited at the General Microbiology Culture Collection Center of China Committee for Culture Collection of Microorganisms under accession number CGMCC No. 8414.

Dependencies are inferred from the claim text.

Mortierella alpine uracil auxotroph with URA5 gene knocked out through homologous recombination, and construction method thereof

Life of this patent

Mortierella alpine uracil auxotroph with URA5 gene knocked out through homologous recombination, and construction method thereof

Life of this patent

Abstract

Claims1