Modified gram positive bacteria and uses thereof
AssigneeIntrexon Actobiotics NV
InventorsLothar Steidler, Karolien Van Huynegem, Klaas Vandenbroucke
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Abstract
The present invention relates to gram positive bacteria with increased stress resistance and/or improved storage characteristics. In particular, the invention relates to gram positive bacterium which accumulate intracellular trehalose. The gram positive bacterium according to the invention lack cellobiose-specific PTS system IIC component (PtcC) activity. The gram positive bacterium may further lack trehalose 6-phosphate phosphorylase (TrePP) activity. The gram positive bacterium may further overexpress trehalose transporters. The invention further relates to compositions comprising such gram positive bacterium as well as methods and uses thereof.
Claims18
A lactic acid bacterium (LAB) or Bifidobacterium, each lacking cellobiose-specific PTS system IIC component (PtcC) activity, wherein a gene encoding an endogenous PtcC of said LAB or said Bifidobacterium has been partially or completely deleted, disrupted, or inactivated so as to be incapable of producing a functional ptcC gene product, and wherein said LAB or said Bifidobacterium is genetically engineered to overexpress a gene encoding a trehalose transporter.
Dependencies are inferred from the claim text.