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US 9,981,239 B2Granted
Devices and methods for oligonucleic acid library synthesis
AssigneeTwist Bioscience Corporation
InventorsWilliam Banyai, Bill James Peck, Andres Fernandez, Siyuan Chen, Pierre Indermuhle, Eugene P. Marsh
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Abstract
Devices and methods for de novo synthesis of large and highly accurate libraries of oligonucleic acids are provided herein. Devices include structures having a main channel and microchannels, where the microchannels have a high surface area to volume ratio. Devices disclosed herein provide for de novo synthesis of oligonucleic acids having a low error rate.
Claims28
A device for synthesizing oligonucleotides, comprising:
- a plate;
- a plurality of main channels, wherein each main channel extends vertically into the plate from an opening on a top side of the plate, and wherein the plurality of main channels comprises more than 250 main channels;
- and
- for each main channel, a plurality of microchannels connected thereto, wherein each microchannel of the plurality of microchannels extends vertically from an opening on a bottom side of the plate to said main channel, and wherein each microchannel of the plurality of microchannels has a surface area to volume ratio of greater than 0.2 um−1.
1 more element
A device for synthesizing oligonucleotides, comprising:
- a silicon plate;
- a plurality of main channels, wherein the plurality of main channels comprises more than 250 main channels, wherein each main channel extends vertically into the silicon plate from an opening on a top side of the silicon plate, and wherein each main channel has a width of 0.5 mm to 2 mm;
- and
- for each main channel, a plurality of microchannels connected thereto, wherein each microchannel of the plurality of microchannels extends vertically from an opening on a bottom side of the silicon plate to said main channel, and wherein each microchannel of the plurality of microchannels comprises a total width that is less than 100 um, a microchannel surface area greater than 10,000 um2, and a maximum width for a narrowest segment of the microchannel of 10 um.
1 more element
Dependencies are inferred from the claim text.