Method and device for carrying out thin-layer chromatography
AssigneeMerck Patent GmbH
InventorMichael Schulz
Life of this patent
Estimating the term…
Abstract
A method for performing thin-layer chromatography applies a liquid sample to a separating layer. A mobile phase is subsequently applied to the separating layer and brought into contact with the liquid sample. After a developmental phase, a thin-layer chromatogram is produced. The liquid sample is taken up from a sample storage container with the aid of an autopipette using a new pipette tip for each sample application operation and applied to the separating layer. A device for performing the method includes a support plate for the accommodation of the separating layer, a first storage container for the accommodation of the sample, and a second storage container for the accommodation of the mobile phase.
Claims12
A method for performing thin-layer chromatography, the method comprising:
- applying at least one liquid sample to a separating layer (14), wherein the separating layer is positioned on a horizontal support plate,
- subsequently applying a mobile phase to the separating layer (14) positioned on the horizontal support plate and bringing the mobile phase into contact with the sample, and
- after a development phase, producing a thin-layer chromatogram wherein the liquid sample is taken up from a sample storage container (11, 12, 13) with the aid of an autopipette (4) using a new pipette tip (8, 9) for each sample application operation and applied to the separating layer (14),
- wherein an area of the separating layer to be evaluated is stamped out using the new pipette tip for each separation operation and fed to an evaluation device.
A device (5) for performing thin-layer chromatography automatically, the device comprising:
- a horizontal support plate (15) for the accommodation of a separating layer (14),
- a first storage container (10, 11, 12, 13) for the accommodation of a sample, and
- a second storage container (16) for the accommodation of a mobile phase,
- wherein the device (5) has an automatic pipetting device (4) which is moved at least over one surface of the horizontal support plate (15) and over the first storage container (10, 11, 12, 13) and the second storage container (16) and has a stock of pipette tips (8, 9), which is taken up successively by the pipetting device (4), used and subsequently detached from the pipetting device (4) again and removed, and
- wherein the horizontal support plate is held in a horizontal position during the mobile phase,
- wherein the device has the pipette tips for separating out an area of the separating layer and for transfer of the separated-out area of the separating layer to an evaluation unit.
Dependencies are inferred from the claim text.