Back to results
US 9,976,941 B2Granted
In situ heat induced antigen recovery and staining method
OwnerLee H. Angrosinventor
Life of this patent
Estimating the term…
Abstract
An automated in situ heat induced antigen recovery and staining method and apparatus for treating a plurality of microscope slides. The process of heat induced antigen recovery and the process of staining the biological specimen on the microscope slide are conducted in the same apparatus, wherein the microscope slides do not need to be physically removed from one apparatus to another. The reaction conditions for treating a slide can preferably be controlled independently, including the individualized application of reagents to each slide and the individualized treatment of each slide.
Claims30
An automated apparatus for treating biological specimens positioned on microscope slides, comprising:
- a first slide support element for supporting a first microscope slide and a second slide support element for supporting a second microscope slide, at least the first slide support element being movable to a treatment position, wherein a first biological specimen positioned on the first microscope slide is treated, while the second slide support element is in a treatment position, wherein a second biological specimen positioned on the second microscope slide is treated;
- a first heating element corresponding to the first slide support element to heat the first microscope slide when in the treatment position thereof and a second heating element corresponding to the second slide support element to heat the second microscope slide when in the treatment position thereof such that the first heating element heats the first microscope slide independently of the heating of the second microscope slide by the second heating element;
- and
- a microprocessor for controlling a treatment protocol performed on the first slide support element and the second slide support element, determining if one of the first biological specimen and the second biological specimen has completed the treatment protocol, and causing the slide support element supporting the microscope slide with the biological specimen that has completed the treatment protocol to be positioned for removal of the microscope slide with the biological specimen that has completed the treatment protocol from the apparatus while the other biological specimen continues to be treated in the treatment position.
1 more element
An automated apparatus for treating biological specimens positioned on microscope slides, comprising:
- a first slide support element for supporting a first microscope slide and a second slide support element for supporting a second microscope slide, at least the first slide support element being movable from a treatment position, wherein a first biological specimen positioned on the first microscope slide is treated, while the second slide support element is in a treatment position, wherein a second biological specimen positioned on the second microscope slide is treated;
- a first heating element corresponding to the first slide support element to heat the first microscope slide when in the treatment position thereof and a second heating element corresponding to the second slide support element to heat the second microscope slide when in the treatment position thereof such that the first heating element heats the first microscope slide independently of the heating of the second microscope slide by the second heating element;
- and
- a microprocessor for controlling a treatment protocol performed on the first slide support element and the second slide support element, determining if one of the first biological specimen and the second biological specimen has completed the treatment protocol, and causing the slide support element supporting the microscope slide with the biological specimen that has completed the treatment protocol to be positioned for removal of the microscope slide with the biological specimen that has completed the treatment protocol from the apparatus while the other biological specimen continues to be treated in the treatment position.
1 more element
An automated apparatus for treating biological specimens positioned on microscope slides, comprising:
- a treatment chamber;
- a first slide support element for supporting a first microscope slide and a second slide support element for supporting a second microscope slide, at least the first slide support element being movable to a treatment position in the treatment chamber, wherein a first biological specimen positioned on the first microscope slide is treated, while the second slide support element is in a treatment position, wherein a second biological specimen positioned on the second microscope slide is treated;
- a first heating element corresponding to the first slide support element to heat the first microscope slide when in the treatment position thereof and a second heating element corresponding to the second slide support element to heat the second microscope slide when in the treatment position thereof such that the first heating element heats the first microscope slide independently of the heating of the second microscope slide by the second heating element;
- and
- a microprocessor for controlling a treatment protocol performed on the first slide support element and the second slide support element, determining if one of the first biological specimen and the second biological specimen has completed the treatment protocol, and causing the slide support element supporting the microscope slide with the biological specimen that has completed the treatment protocol to be positioned for removal of the microscope slide with the biological specimen that has completed the treatment protocol from the apparatus while the other biological specimen continues to be treated in the treatment position.
2 more elements
An automated apparatus for treating biological specimens positioned on microscope slides, comprising:
- a treatment chamber;
- a first slide support element for supporting a first microscope slide and a second slide support element for supporting a second microscope slide, at least the first slide support element being movable from a treatment position in the treatment chamber, wherein a first biological specimen positioned on the first microscope slide is treated, while the second slide support element is in a treatment position, wherein a second biological specimen positioned on the second microscope slide is treated;
- a first heating element corresponding to the first slide support element to heat the first microscope slide when in the treatment position thereof and a second heating element corresponding to the second slide support element to heat the second microscope slide when in the treatment position thereof such that the first heating element heats the first microscope slide independently of the heating of the second microscope slide by the second heating element;
- and
- a microprocessor for controlling a treatment protocol performed on the first slide support element and the second slide support element, determining if one of the first biological specimen and the second biological specimen has completed the treatment protocol, and causing the slide support element supporting the microscope slide with the biological specimen that has completed the treatment protocol to be positioned for removal of the microscope slide with the biological specimen that has completed the treatment protocol from the apparatus while the other biological specimen continues to be treated in the treatment position.
2 more elements
Dependencies are inferred from the claim text.