System and method for the detection of allergens
AssigneeDH Technologies Development Pte. Ltd.
InventorsStephen J. Lock, Subhasish Purkayastha, Brian L. Williamson, Keling Dong
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Abstract
Methods and systems for detecting allergens using mass spectrometry are provided herein. In some aspects, a sample can be screened for the presence or quantity of ovalbumin, lysozyme, casein (isoform S1 and S2), lactoglobulin, high and low glutens, wheat, rye, oats, barley, mustard, sesame, and various types of nuts including macadamia, pistachio, brazil, walnuts, peanuts and hazelnuts by detecting one or more peptides specific to the allergen of interest using selected MRM transitions.
Claims6
A method of detecting casein in a sample said method comprising:
- adding a proteolytic enzyme to the sample to lyse at least a portion of the casein present in the sample, into a plurality of peptides;
- and
- utilizing liquid chromatography tandem mass spectrometry (LC-MS/MS) to determine whether at least one peptide is in the sample
- wherein said determining whether at least one peptide is in the sample comprises monitoring at least one precursor-product ion pair transition with specified m/z value associated with a specific amino acid sequence selected from the group consisting of:
- i) SEQ ID NO:
- 14, m/z value of 416/488, 416/587, or 416/702;
- and
- ii) SEQ ID NO:
- 15, m/z value of 634/771, or 634/935.
A method of detecting lactoglobulin in a sample, said method comprising:
- adding a proteolytic enzyme to the sample to lyse at least a portion of the lactoglobulin present in the sample, into a plurality of peptides;
- and
- utilizing liquid chromatography tandem mass spectrometry (LC-MS/MS) to determine whether at least one peptide is in the sample, wherein
- said determining whether at least one peptide is in the sample comprises monitoring at least one precursor-product ion pair transition with specified m/z value associated with a specific amino acid sequence SEQ ID NO:
- 27, m/z value of 623/573, 623/918, 623/819, or 623/1047.
Dependencies are inferred from the claim text.