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US 9,970,042 B2Granted
Enzymatic process for the preparation of testosterone and esters thereof
AssigneeF.I.S. Fabbrica Italiana Sintetici S.p.A.
InventorsAlfredo Paio, Stefano Fogal, Riccardo Motterle
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Abstract
The present invention relates to a new process for the preparation of testosterone by means of enzymatic hydrolysis of testosterone esters.
Claims12
Process for the preparation of testosterone of formula (I):
- by performing an enzymatic hydrolysis of a testosterone ester of formula (II) in an aqueous buffer solution comprising an enzyme and the compound of formula (II):
- wherein R is a C1-C9 linear or branched alkyl group or is a C3-C9 cyclic or branched cyclic alkyl group, wherein the enzyme is a Humicola insolens lipase and DNA sequence encoding the Humicola insolens lipase is genetically modified or not genetically modified, or wherein the enzyme is Candida antarctica lipase A.
Process according to claim from 1, wherein in the testosterone esters of formula (II) R is a C1-C6 linear or branched alkyl group, or is a C7 branched cyclic alkyl group.
Process according to claim from 1, wherein the process is carried out at a temperature between about 35° C. and about 70° C.
Dependencies are inferred from the claim text.