RNA-guided human genome engineering
AssigneePresident and Fellows of Harvard College
InventorsGeorge M. Church, Prashant Mali, Luhan Yang
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Abstract
A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.
Claims7
A method of visualizing a target nucleic acid sequence in a eukaryotic cell comprising providing to the eukaryotic cell a guide RNA complementary to the target nucleic acid sequence, and providing to the cell a Cas 9 protein having inactive nuclease domains and having a fluorescent molecule attached thereto, wherein the guide RNA and the Cas 9 protein having the fluorescent molecule attached thereto form a complex at the target nucleic acid sequence and is visualized.
Dependencies are inferred from the claim text.