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US 9,970,018 B2Granted
Biosynthesis of human milk oligosaccharides in engineered bacteria
AssigneeGlycosyn LLC
InventorsMassimo Merighi, John M. McCoy, Matthew Ian Heidtman
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Abstract
The invention provides compositions and methods for engineering bacteria to produce fucosylated oligosaccharides, and the use thereof in the prevention or treatment of infection.
Claims28
A method for producing a fucosylated oligosaccharide in a bacterium, comprising
- providing an isolated E. coli bacterium comprising,
- (i) a deletion or functional inactivation of an endogenous β-galactosidase gene;
- (ii) an exogenous functional β-galactosidase gene comprising a detectable level of β-galactosidase activity that is reduced compared to that of a wild-type E. coli bacterium, wherein the level of β-galactosidase activity comprises between 0.05 and 200 units;
- (iii) an inactivating mutation in a colanic acid synthesis gene;
- and
- (iv) an exogenous lactose-accepting fucosyltransferase gene;
- culturing said bacterium in the presence of lactose;
- and
- retrieving a fucosylated oligosaccharide from said bacterium or from a culture supernatant of said bacterium.
6 more elements
Dependencies are inferred from the claim text.