Aspergillus fumigatus cellulolytic enzyme compositions and uses thereof

An enzyme composition comprising a recovered fermentation broth of a recombinant Trichoderma host cell, wherein the recombinant Trichoderma host cell comprises polynucleotides encoding:

• (i) an Aspergillus fumigatus cellobiohydrolase I;
• (ii) an Aspergillus fumigatus cellobiohydrolase II;
• (iii) an Aspergillus fumigatus beta-glucosidase;
• (iv) an Aspergillus fumigatus GH61 polypeptide having cellulolytic enhancing activity;
• (v) an Aspergillus fumigatus xylanase;
• and (vi) an Aspergillus fumigatus beta-xylosidase;
• or homologs thereof;
• wherein the Aspergillus fumigatus cellobiohydrolase I or homolog thereof is selected from the group consisting of:
• (i) a cellobiohydrolase I comprising the mature polypeptide of SEQ ID NO:
• 2;
• (ii) a cellobiohydrolase I comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
• 2;
• (iii) a cellobiohydrolase I encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
• 1;
• and
• (iv) a cellobiohydrolase I encoded by a polynucleotide that hybridizes under very high stringency conditions with the mature polypeptide coding sequence of SEQ ID NO:
• 1 or the full-length complement thereof;
• wherein the Aspergillus fumigatus cellobiohydrolase II or homolog thereof is selected from the group consisting of:
• (i) a cellobiohydrolase II comprising the mature polypeptide of SEQ ID NO:
• 4;
• (ii) a cellobiohydrolase II comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
• 4;
• (iii) a cellobiohydrolase II encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
• 3;
• and
• (iv) a cellobiohydrolase II encoded by a polynucleotide that hybridizes under very high stringency conditions with the mature polypeptide coding sequence of SEQ ID NO:
• 3 or the full-length complement thereof;
• wherein the Aspergillus fumigatus beta-glucosidase or homolog thereof is selected from the group consisting of:
• (i) a beta-glucosidase comprising the mature polypeptide of SEQ ID NO:
• 6;
• (ii) a beta-glucosidase comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
• 6;
• (iii) a beta-glucosidase encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
• 5;
• and
• (iv) a beta-glucosidase encoded by a polynucleotide that hybridizes under very high stringency conditions with the mature polypeptide coding sequence of SEQ ID NO:
• 5 or the full-length complement thereof;
• wherein the Aspergillus fumigatus GH61 polypeptide having cellulolytic enhancing activity or homolog thereof is selected from the group consisting of:
• (i) a GH61 polypeptide having cellulolytic enhancing activity comprising the mature polypeptide of SEQ ID NO:
• 8;
• (ii) a GH61 polypeptide having cellulolytic enhancing activity comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
• 8;
• (iii) a GH61 polypeptide having cellulolytic enhancing activity encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
• 7;
• and
• (iv) a GH61 polypeptide having cellulolytic enhancing activity encoded by a polynucleotide that hybridizes under very high stringency conditions with the mature polypeptide coding sequence of SEQ ID NO:
• 7 or the full-length complement thereof;
• wherein the Aspergillus fumigatus xylanase or homolog thereof is selected from the group consisting of:
• (i) an Aspergillus fumigatus xylanase comprising the mature polypeptide of SEQ ID NO:
• 14, SEQ ID NO:
• 16, or SEQ ID NO:
• 18;
• (ii) a xylanase comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
• 14, SEQ ID NO:
• 16, or SEQ ID NO:
• 18;
• (iii) a xylanase encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
• 13, SEQ ID NO:
• 15, or SEQ ID NO:
• 17;
• and
• (iv) a xylanase encoded by a polynucleotide that hybridizes under very high stringency conditions with the mature polypeptide coding sequence of SEQ ID NO:
• 13, SEQ ID NO:
• 15, or SEQ ID NO:
• 17;
• or the full-length complement thereof;
• wherein the Aspergillus fumigatus beta-xylosidase or homolog thereof is selected from the group consisting of:
• (i) a beta-xylosidase comprising the mature polypeptide of SEQ ID NO:
• 20;
• (ii) a beta-xylosidase comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
• 20;
• (iii) a beta-xylosidase encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
• 19;
• and
• (iv) a beta-xylosidase encoded by a polynucleotide that hybridizes under very high stringency conditions with the mature polypeptide coding sequence of SEQ ID NO:
• 19 or the full-length complement thereof;
• and
• wherein very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide, and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 70° C.