Merocyanine-based compounds, and dyes, kits and contrast medium compositions for labelling biomolecules comprising the same

A merocyanine-based compound having a structure represented by the following formula 1:

• wherein
• Ar is a substituted or unsubstituted aromatic ring;
• Y1 and Y2 are each independently selected from sulfur, oxygen, selenium, NR8 and —CR8=CR9-;
• R1 to R9 are each independently selected from the group consisting of hydrogen, deuterium, substituted or unsubstituted C1-C10 alkyl, substituted or unsubstituted C2-C10 heteroalkyl containing at least one heteroatom, substituted or unsubstituted C2-C10 alkenyl, substituted or unsubstituted C2-C10 alkynyl, substituted or unsubstituted C1-C10 alkoxy, substituted or unsubstituted aryloxy, substituted or unsubstituted C1-C10 haloalkyl, halogen, cyano, hydroxyl, substituted or unsubstituted amino, substituted or unsubstituted amide, carbamate, sulthydryl, nitro, carboxyl, carboxylate, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted aralkyl, quaternary ammonium, phosphoric acid, phosphate, phosphonate, —COR10, aldehyde, —COOR10, acyl chloride, sulfonic acid, sulfonate, polyalkylene oxide, and -L-Z functional groups;
• when Ra, wherein a is an integer selected from 1 to 9, is a —COR10 group or a —COOR10 group, R10 is selected from the group consisting of substituted or unsubstituted C1-C10 alkyl, substituted or unsubstituted C2-C10 heteroalkyl containing at least one heteroatom, substituted or unsubstituted C2-C10 alkenyl, substituted or unsubstituted C2-C10 alkynyl, substituted or unsubstituted C1-C10 alkoxy, substituted or unsubstituted C1-C10 haloalkyl, and substituted or unsubstituted C1-C10 aminoalkyl;
• when Rb, wherein b is an integer selected from 1 to 10, is substituted, any carbon or terminal carbon in the functional groups may be substituted with at least one substituent selected from the group consisting of sulfonic acid, sulfonate, ketone, aldehyde, carboxylic acid, carboxylate, phosphoric acid, phosphate, phosphonate, acyl chloride, polyalkylene oxide, quaternary ammonium salt, ester, and amide;
• m is an integer of 1 to 3;
• L is a linker comprising 3 to 150 non-hydrogen atoms;
• Z is a fluorescent moiety capable of generating a fluorescent signal, or has a structure represented by formula 1;
• and
• wherein the structure represented by the formula 1 has one or two -L-Z functional groups.