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US 9,964,552 B2Granted
Mass spectrometric identification and/or quantitation of catecholamines using aminopyrazoles
AssigneeDH Technologies Development Pte. Ltd.
InventorsSubhakar Dey, Subhasish Purkayastha
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Abstract
A method is described for mass spectrometric analysis, detection and quantification of catecholamines. The methods can comprise reacting the catecholamines with a 4-aminoantipyrine reagent and detecting and/or quantifying the adduct produced by the reaction. The methods can also allow for multiplexing. Compounds formed by the reactions are also provided.
Claims8
A method of detecting the presence of one or more catecholamines in a sample, the method comprising:
- a) reacting the sample with a 4-aminoantipyrine reagent, or a heavy atom derivative thereof, under conditions sufficient to react with a catecholamine, if any, present in the sample to form a 4-aminoantipyrine-reagent-catecholamine adduct, or a heavy atom derivative thereof;
- b) obtaining a mass spectrum of said reacted sample;
- and
- c) analyzing the mass spectrum to determine whether the sample contains a 4-aminoantipyrine-reagent-catecholamine adduct or an ionic fragment thereof, wherein the 4-aminoantipyrine reagent is a compound of Formula I or a heavy atom derivative thereof:
- wherein
- R1
- y is 1-6;
- and
- X is an anion.
6 more elements
A method of detecting the presence of one or more catecholamines in a sample, the method comprising:
- a) reacting the sample with a 4-aminoantipyrine reagent, or a heavy atom derivative thereof, under conditions sufficient to react with a catecholamine, if any, present in the sample to form a 4-aminoantipyrine-reagent-catecholamine adduct, or a heavy atom derivative thereof;
- b) obtaining a mass spectrum of said reacted sample;
- and
- c) analyzing the mass spectrum to determine whether the sample contains a 4-aminoantipyrine-reagent-catecholamine adduct or an ionic fragment thereof, wherein the aminoantipyrine-catecholamine adduct is a compound of Formula II, or a heavy atom derivative thereof:
- wherein
- R4 is H or
- R5 is
- z is 1-6;
- and
- Q is a halogen.
7 more elements
Dependencies are inferred from the claim text.