Method for producing carotenoids each having 50 carbon atoms
AssigneeNational University Corporation Chiba University
InventorsDaisuke Umeno, Maiko Furubayashi, Norihiko Misawa, Shinichi Takaichi
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Abstract
The present invention provides a method of producing a carotenoid having 50 carbon atoms which comprises culturing, in a medium, a cell transformed with a mutant phytoene desaturase gene and obtaining the carotenoid having 50 carbon atoms from the culture. The mutant phytoene desaturase gene has an introduced mutation to encode a mutant phytoene desaturase having an enhanced activity to desaturate a carotenoid backbone compound of 50 carbon atoms.
Claims2
A method of producing a carotenoid having 55 carbon atoms, comprising:
- culturing, in a medium, a cell transformed with a mutant diapophytoene synthase gene and a mutant farnesyl diphosphate synthase gene;
- and
- obtaining the carotenoid having 55 carbon atoms from a culture after the culturing,
- wherein the transformation is performed by a plasmid having the nucleotide sequence of SEQ ID NO:
- 26 that contains the mutant diapophytoene synthase gene and a plasmid having the nucleotide sequence of SEQ ID NO:
- 25 that contains the mutant farnesyl diphosphate synthase gene.
A method of producing a carotenoid having 60 carbon atoms, comprising:
- culturing, in a medium, a cell transformed with a mutant diapophytoene synthase gene and a mutant hexaprenyl diphosphate synthase gene;
- and
- obtaining the carotenoid having 60 carbon atoms from a culture after the culturing,
- wherein the transformation is performed by a plasmid having the nucleotide sequence of SEQ ID NO:
- 26 that contains the mutant diapophytoene synthase gene and a plasmid having the nucleotide sequence of SEQ ID NO:
- 24 that contains the mutant farnesyl diphosphate synthase gene.
Dependencies are inferred from the claim text.