Method for isolation and purification of epithelial stem cells from skin
AssigneePell Cell Medicals NV
InventorsSarah Y. Broeckx, Jan H. Spaas
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Abstract
The current invention concerns a method for obtaining a cellular composition comprising epithelial stem cells (EpSCs) from mammalian skin, whereby said composition comprises at least 90% of viable EpSCs, comprising the steps of: —obtaining a mammalian skin sample; —obtaining a cell suspension from said skin sample by performing at least one enzymatic dissociation step; and —culturing said cell suspension under low-attachment conditions. Preferably cellular composition comprises epithelial stem cells derived from the epidermal layer. In a second aspect, the current invention provides for a cellular composition obtained by the method according to the invention.
Claims10
A method for obtaining a cellular composition comprising epithelial stem cells (EpSCs) derived from the epidermal layer, whereby said composition comprises at least 90% of viable EpSCs, comprising the steps of:
- obtaining a mammalian skin sample;
- at least partially separating the epidermal layer from the dermal layer;
- obtaining a cell suspension from said epidermal layer by performing at least one enzymatic dissociation step;
- culturing said cell suspension under low-attachment conditions;
- selecting non-adherent cells;
- and
- amplifying the selected cells under adherent conditions.
Dependencies are inferred from the claim text.