Detection apparatus having a microfluorometer, a fluidic system, and a flow cell latch clamp module

A detection apparatus, comprising:

• a microfluorometer comprising an objective, an excitation radiation source, and a detector, the microfluorometer configured for wide-field image detection;
• a fluidic system for delivering reagents from a reagent cartridge to a flow cell, the fluidic system comprising:
• a manifold body having a plurality of fluidic channels configured for fluid communication between the reagent cartridge and an inlet of the flow cell;
• a plurality of reagent sippers extending downward from ports in the manifold body, each of the reagent sippers configured to be placed into a reagent reservoir of the reagent cartridge so that liquid reagent can be drawn from the reagent reservoir into the reagent sipper;
• and
• a valve configured to mediate fluid communication between the reagent reservoirs and the inlet of the flow cell, the fluidic channels fluidly connecting the plurality reagent sippers to the valve;
• and
• a flow cell latch clamp module, comprising:
• a clamp base;
• a clamp cover for holding the flow cell, the clamp cover rotatably coupled to the clamp base, the clamp cover comprising a rotatable arm and a clamp manifold, wherein the rotatable arm extends through a cavity of a body of the clamp cover, and wherein the rotatable arm is directly connected to the clamp manifold, the clamp manifold having at least one port configured to be in flow communication with the flow cell;
• a first biasing member that provides a rotating force for rotating the rotatable arm away from the clamp base;
• and
• a second biasing member, the second biasing member engaging the rotatable arm to facilitate locating the clamp manifold,
• wherein the objective is configured to direct excitation radiation from the radiation source to the flow cell and to direct emission from the flow cell to the detector, and wherein the microfluorometer is movable to acquire wide-field images of different areas of an inner surface of the flow cell.