Device and method for detection of analytes
AssigneeXcellcure, LLC
InventorsLeah Amir, Gary Anderson
Life of this patent
Estimating the term…
Abstract
A detection device and associated systems and methods for detecting analytes from a multiplex reaction are described. In particular, a device for conducting at least one detection reaction using a modified ELISA method including a surface with a detection region and a reference region, a detection sensor, and a light source. The detection device may include a complementary metal-oxide-semiconductor (CMOS) image sensor. The device may be used to measure and report discrete quantities or combinations of discrete analytes, providing information to aid in the prognosis and/or diagnosis of altered states of health in vertebrates.
Claims9
A device for the detection of one or more analytes in a sample obtained from a subject, the device comprising:
- a) a surface to conduct at least one detection reaction, the surface comprising:
- a detection region comprising one or more epitope binding agents, wherein each epitope binding agent is configured to bind exclusively to one of the at least one analytes;
- and
- a reference region, wherein the detection region is separate from the reference region;
- b) one or more detection sensors to sense light associated with the detection region;
- c) one or more reference sensors to sense light associated with the reference region;
- and
- wherein the one or more detection sensors and the one or more reference sensors are operated using a balanced sensor method.
A system for detecting one or more analytes in a sample and diagnosing a condition, the system comprising:
- a) a device comprising:
- a surface to conduct at least one detection reaction, the surface comprising a detection region and a reference region, wherein the detection region is separate from the reference region;
- one or more detection sensors to sense light associated with the detection region;
- one or more reference sensors to sense light associated with the reference region;
- and
- at least one light source to illuminate the detection region and the reference region;
- b) a computing device comprising one or more processors;
- c) a memory comprising:
- a calibration database comprising one or more calibration sets, each calibration set comprising a conversion between a combined sensor readout and a concentration of an analyte;
- a diagnostic database comprising one or more threshold values and one or more diagnostic rules;
- a CRM encoded with an analyte detection application comprising a plurality of modules executable by the one or more processors, the modules comprising:
- a control module to control the operation of the device and to coordinate the function of the plurality of modules;
- a balanced detection module to control the operation of the one or more sensors and/or light sources of the device;
- an analyte detection module to process combined output signals received from the balanced detection module;
- a post-processing module to further analyze the analyte concentrations calculated by the analyte detection module;
- a diagnostic module to compare the entries of the diagnostic array and to determine a condition of a patient according to one or more diagnostic rules defined within the diagnostic database stored in memory;
- and
- a GUI module to generate one or more forms to receive input to the system and/or display output from the system;
- and
- d) a display.
Dependencies are inferred from the claim text.