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US 9,958,452 B2Granted
Highly fluorogenic protein labelling agents
AssigneeUniversity of Ottawa
InventorsJeffrey Keillor, Yingche Chen
Life of this patent
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Abstract
There are provided fluorogenic labelling agents comprising a dimaleimide core connected to a BODIPY-based fluorophore, processes for preparation thereof, and uses thereof for labelling and/or detection of specific protein targets. Fluorogenic labelling agents having an excitation wavelength that can be visualized in the green or red channels of fluorescence microscopes are provided. In some embodiments, fluorogenic labelling agents comprising a compound having the structure of Formula I or II, and salts thereof, are described.
Claims23
A fluorogenic labelling agent comprising a compound of Formula I, or a salt thereof:
- wherein:
- R1, R2, R3, R4, R5, and R6 are independently selected from hydrogen, substituted or unsubstituted alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, cycloalkynyl, aryl, alkaryl, arylalkyl, carboxy alkyl, heterocyclic, and heteroaryl;
- and
- X and Y are independently R7 or OR8, wherein R7 is selected from hydrogen, halogen, and substituted or unsubstituted alkyl and R8 is substituted or unsubstituted alkyl.
1 more element
Dependencies are inferred from the claim text.