Back to results
US 9,958,450 B2Granted
L-glucose derivatives having fluorescent chromophore
AssigneeHirosaki University
InventorsKatsuya Yamada, Tadashi Teshima, Toshihiro Yamamoto
Life of this patent
Estimating the term…
Loading patent…
Loading patent…
AssigneeHirosaki University
InventorsKatsuya Yamada, Tadashi Teshima, Toshihiro Yamamoto
Estimating the term…
An object of the present invention is to provide a method for accurately evaluating the specific incorporation of D-glucose into cells. The present invention as a means for achieving the object is characterized by comprising contacting a D-glucose derivative that has a fluorescent chromophore in the molecule and is specifically incorporated into cells and an L-glucose derivative that has a fluorescent chromophore in the molecule with different cells in the same cell strain to be evaluated, respectively, comparing the fluorescence emitted by the D-glucose derivative that has a fluorescent chromophore in the molecule and is specifically incorporated into cells with the fluorescence emitted by the L-glucose derivative that has a fluorescent chromophore in the molecule, and evaluating the specific incorporation of D-glucose into cells relative to L-glucose by taking the difference between the two kinds of fluorescence intensities.
An L-glucose derivative, characterized by having a fluorescent chromophore in the molecule, wherein the fluorescent chromophore is selected from the group consisting of an N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino group, a 7-(N,N-dimethylaminosulfonyl)benz-oxa-1,3-diazol-4-yl)amino group and sulforhodamine.
A method of producing an L-glucose derivative that has a fluorescent chromophore in the molecule, comprising the step of:
Dependencies are inferred from the claim text.