Method for enzyme-mediated signal amplification
AssigneeDako Denmark A/S
InventorsJesper Lohse, Galina Skladchikova
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Abstract
The present invention relates to methods and compounds for enzyme-mediated amplification of target-associated signals for visualization of biological or chemical targets in samples, wherein the targets are immobilized, in particular the invention relates to the oxidoreductase-mediated signal amplification for visualization of targets in samples comprising cells. The methods of the invention are particular useful for qualitative and quantitative evaluation of biological markers relating to medical diagnostic and therapy.
Claims15
A method for detection of an immobilized target in a sample, wherein the target inherently comprises or is linked to an enzyme with oxidoreductase enzymatic activity, comprising incubating a sample comprising the target in an aqueous solution comprising:
- (i) a first substrate of said enzymatic activity and
- (ii) a second substrate of said enzymatic activity,
- wherein the first substrate is alpha-cyano-4-hydroxycinnamic acid (ACHCA) and the second substrate is a conjugate molecule comprising one or more compounds capable of serving as substrates of said oxidoreductase enzymatic activity and a detectable label, and
- detecting the detectable label of the second substrate.
Dependencies are inferred from the claim text.