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US 9,958,436 B2Granted
Determination of cell chirality
AssigneeThe Trustees of Columbia University in the City of New York
InventorsGordana Vunjak-Novakovic, Leo Qun Wan
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Abstract
Methods for determining cell chirality using micropatterned substrates are disclosed. Also provided are methods for diagnosing diseases such as genetic diseases or cancer by comparing the chirality of sample cells from a subject with normal cells, and determining a difference in chirality between the sample cells and normal cells.
Claims6
A method for determining chirality of a cell comprising:
- (a) applying cells to a surface of a micropatterned substrate;
- wherein,
- the micropatterned substrate comprises an x-axis, a y-axis, an inner boundary, and an outer boundary, wherein the surface is disposed between the inner boundary and the outer boundary;
- the inner boundary or the outer boundary is at least partially or substantially perpendicular to the x-axis and at least partially or substantially parallel with the y-axis;
- (b) culturing the cells on the micropatterned substrate for an amount of time sufficient for a portion of the cells to contact the inner boundary or the outer boundary;
- (c) measuring an angular deviation away from the y-axis for the portion of cells, wherein the angular deviation is a positive angular deviation or a negative angular deviation, wherein the positive angular deviation is classified as a counterclockwise (CCW) alignment and the negative angular deviation is classified as a clockwise (CW) alignment, and wherein the CCW alignment and CW alignment denotes the chirality of the portion of the cells.
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Dependencies are inferred from the claim text.