Method and system for in vitro developmental toxicity testing
AssigneeAgency for Science, Technology and Research
InventorsHanry Yu, Yi-Chin Toh, Jiangwa Xing
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Abstract
A method and system of in vitro developmental toxicity testing comprising the steps of micropatterning an extracellular matrix; growing embryonic stem cells on the micropatterned extracellular matrix in the presence of mesoendodermal induction and testing for change of the geometrical mesoendoderm structure in the presence or absence of a test compound wherein (1) a decrease in mesoendodermal differentiation and/or (2) a change in morphology of the geometrical mesoendoderm structure in the presence of the test compound compared to cells in the absence of the test compound indicates that the test compound is a developmental toxic agent.
Claims17
An in vitro method of testing developmental toxicity comprising the steps of:
- (a) differentiating a colony of pluripotent stem cells on a micropatterned extracellular matrix in the presence of a mesoendodermal induction medium and a test compound, wherein the differentiation of the colony coordinates cell movement and spatially patterned mesoendodermal differentiation and said differentiation forms a geometrical mesoendoderm structure in the colony;
- and
- (b) testing for a change in the spatially patterned differentiation of the geometrical mesoendoderm structure, wherein a disruption in the spatially patterned differentiation of the geometrical mesoendoderm structure compared to an untreated control colony of pluripotent stem cells indicates that the test compound is a developmentally toxic compound.
Dependencies are inferred from the claim text.