Endotoxin detection systems and detection methods thereof

An endotoxin detection method comprising a polydisperse particle size distribution detection and a monodisperse particle size distribution detection utilizing a system comprising a laser source, a light intensity regulator, a scanning mode regulator, a short focal length lens, a grating, a long focal length lens, a detection cell, a scattered light receiver, a photoelectric converter, a signal amplifier, a signal processor, a data collector, a quasi-quantitative operator, and a data display, wherein light output from the laser source passes sequentially through the short focal length lens, the grating, and the long focal length lens, and then into the detection cell;

• transmitted light from the detection cell is received by the scattered light receiver, and then enters the photoelectric converter;
• and a signal output from the photoelectric converter is passed sequentially to the signal amplifier, the signal processor, and the data collector, and then to the quasi-quantitative operator for detecting the concentration limits of endotoxin;
• and wherein all of the signal amplifier, the signal processor, the data collector, and the quasi-quantitative operator have an output connected via the light intensity regulator to the laser source, the output of the quasi-quantitative operator is also connected via the scanning mode regulator to the laser source, and the data display is connected to the output of the quasi-quantitative operator , wherein the polydisperse particle size distribution detection comprises:
• Step 1-1:
• adjusting the intensity of the laser source;
• Step 1-2:
• adjusting the scanning mode regulator, and setting the laser source in a step scan mode;
• Step 1-3:
• preparing a series of concentrations of standard endotoxin solutions, and conducting polydisperse particle size distribution detection on each concentration of the standard endotoxin solution;
• Step 1-4:
• detecting the concentration limits of each concentration of the standard endotoxin solution by setting the detection parameters in the quasi-quantitative operator;
• and
• Step 1-5:
• conducting polydisperse particle size distribution detection on a sample by setting the detection parameters in the quasi-quantitative operator as desired by the sample, wherein when the detection result shows that no particle size distribution peak of endotoxin appears in the range of 20-300 nm, the concentration of endotoxin in the sample is below the corresponding concentration limit, or otherwise, the concentration of endotoxin in the sample is above the corresponding concentration limit;
• and
• the monodisperse particle size distribution detection comprises:
• Step 2-1:
• adjusting the intensity of the laser source;
• Step 2-2:
• adjusting the scanning mode regulator, and setting the laser source in a full scan mode;
• Step 2-3:
• preparing a series of concentrations of standard endotoxin solutions, and conducting monodisperse particle size distribution detection on each concentration of the standard endotoxin solution;
• Step 2-4:
• detecting the concentration limits of each concentration of the standard endotoxin solution by setting the detection parameters in the quasi-quantitative operator;
• and
• Step 2-5:
• conducting monodisperse particle size distribution detection on a sample by setting the detection parameters in the quasi-quantitative operator as desired by the sample, wherein when the detection result shows that the particle size distribution peak of endotoxin appears below 20 nm, the concentration of endotoxin in the sample is below the corresponding concentration limit, or otherwise, the concentration of endotoxin in the sample is above the corresponding concentration limit.