Method for dry packing chromatography columns
AssigneeGE Healthcare BioProcess R&D AB
InventorsKlaus Gebauer, Elisabeth Hallgren, Raf Lemmens, Ola Lind, Bjorn Lundgren, Jamil Shanagar, Kajsa Stridsberg-Friden, Helena Widegren
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Abstract
The invention provides a packing method for high efficiency chromatography columns starting from dry swellable particles, as well as columns packed by the method and the use of the columns in separation of biomolecules. In the packing method, an amount of dry swellable particles sufficient to give a swollen volume in a liquid of about 105-120% of the column chamber volume is transferred to the column, the column is closed and the liquid is provided to the column.
Claims8
A method for dry packing a chromatography column with dry swellable particles comprising the steps of:
- a) determining a liquid uptake Vs/md of the dry swellable particles from a separate sample of particles using a weight of the dry swellable particles thereof, and from the liquid uptake Vs/md deciding a weight amount of dry swellable particles to be transferred to a column chamber in the chromatography column, wherein the weight amount of dry swellable particles gives a swollen volume Vs in a liquid of 105-120% of a column chamber volume;
- b) transferring to a flexible container the weight amount of dry swellable particles as determined, based on the liquid uptake, the flexible container being configured to expand to fit the column chamber upon swelling of the dry swellable particles;
- c) transferring the flexible container to the column chamber;
- d) vibrating the chromatography column or injecting gas from the bottom end of the chromatography column;
- and
- e) providing, after vibrating the chromatography column or injecting gas, the liquid to the column chamber upon closing the chromatography column.
Dependencies are inferred from the claim text.