Analyte detection using magnetic hall effect
AssigneeThe General Hospital Corporation
InventorsRalph Weissleder, Hakho Lee, David Issadore
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Abstract
Determining a presence of a target analyte in a fluid sample includes mixing multiple magnetic particles with the fluid sample, in which the magnetic particles are each bound to one or more binding moieties that specifically bind to the target analyte, flowing the fluid sample containing the magnetic particles through a fluidic channel, exposing the fluid sample in the fluidic channel to a magnetic field, measuring a signal from a Hall effect sensor while the fluid sample flows through the fluidic channel, and determining whether the target analyte is present in the fluid sample when the measured signal is in a first range of values.
Claims13
A target analyte detection and analysis device comprising:
- a substrate comprising a plurality of Hall effect sensors;
- a fluidic channel arranged on a surface of the substrate and extending over a detection area of the plurality of Hall effect sensors, the fluidic channel having a length along which a fluid sample flows, a width, and a height,
- wherein the plurality of Hall effect sensors is arranged in multiple rows, each row comprising multiple Hall effect sensors, and each row being located at a different longitudinal position along the length of the fluidic channel, and
- wherein the Hall effect sensors within each row of the multiple rows are laterally staggered along the width of the fluidic channel relative to Hall effect sensors within an adjacent row of the multiple rows, and
- wherein the fluidic channel comprises
- a first input port at a first end of the fluidic channel to receive the fluid sample;
- a second input port at the first end of the fluidic channel to receive a sheath fluid;
- and
- a third input port at the first end of the fluidic channel to receive the sheath fluid, wherein the second input port and the third input port are positioned on opposite sides of the first input port to enable the formation of a sheath flow of the sheath fluid around the fluid sample;
- a magnet arranged to generate a magnetic field in the fluidic channel;
- a plurality of flow focusing structures arranged on a top surface of the fluidic channel and configured to work in conjunction with the sheath flow to direct target analytes in the fluid sample towards the center and bottom of the fluidic channel and closer to the Hall effect sensors;
- and
- control electronics coupled to the plurality of Hall effect sensors, wherein the control electronics are configured to cause the device to:
- (a) detect signals from the plurality of Hall effect sensors;
- (b) determine a presence of a target analyte in the fluidic channel based on the signals;
- and
- (c) determine one or more of:
- (i) a size of a target analyte,
- (ii) a number of magnetic particles bound to a target analyte,
- (iii) a number of each magnetic particle type per cell,
- (iv) a density of a surface marker on a target analyte, and
- (v) a level of expression of a biomarker on the target analyte.
Dependencies are inferred from the claim text.