Method for the detection in biological materials of viruses with lymphotropism properties in low concentrations
AssigneeOvik Leonardovich Mkrtchyan
InventorNariman Gulyamov
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Abstract
Methods and techniques to increase the reliability of detecting virus infections, particularly lymphotropism, to eliminate false negative reactions in testing blood for the presence of lymphotropic viruses during enzyme immunoassay (EIA) and polymerase chain reaction (PCR) testing, and to better detect viruses with lymphotropism in biological materials having a concentration of virus particles lower than the sensitivity threshold of existing EIA and PCR methods, thereby making the techniques of the present invention more reliable.
Claims22
A method for the detection of viruses with lymphotropism properties in a biological material with a virus concentration below enzyme immunoassay (EIA) and polymerase chain reaction (PCR) testing sensitivity thresholds, comprising:
- combining an equal amount of a biological material from a patient suspected of infection by a lymphotropic virus with a lymphocyte suspension from a healthy human subject to form an admixture thereof,
- wherein said biological material of said patient suspected of infection, when subjected to said EIA or said PCR testing, indicates no infection by a lymphotropic virus;
- incubating the admixture;
- washing-out the lymphocytes from the plasma portion in the admixture;
- removing lymphocyte membranes from the admixture, separating the lymphocyte cytoplasm content, thereby concentrating the lymphocyte cytoplasm content;
- and
- detecting the presence or absence of the a virus RNA or DNA in the lymphocyte cytoplasm content,
- wherein the presence of the virus RNA or DNA in the lymphocyte cytoplasm content, with the earlier non-detection of infection, indicates the preserved viability of viruses in said biological sample of said patient, and
- wherein the absence of the virus RNA or in the lymphocyte cytoplasm content indicates the loss of virus viability in said biological sample of said patient,
- whereby viruses with lymphotropism properties with a low virus concentration are detected in biological materials.
A method for the detection of viruses with lymphotropism properties in a biological material with a virus concentration below enzyme immunoassay (EIA) and polymerase chain reaction (PCR) testing sensitivity thresholds, comprising:
- collecting a biological material from a patient suspected of infection by a lymphotropic virus,
- wherein said biological material of said patient suspected of infection, when subjected to said EIA or said PCR testing, indicates no infection by a lymphotropic virus;
- obtaining a lymphocyte suspension from a blood sample of at least one healthy human subject;
- combining an equal amount of said biological material with said lymphocyte suspension to form an admixture thereof;
- stirring and incubating the admixture;
- washing-out the lymphocytes from the plasma portion in the admixture;
- removing lymphocyte membranes from the admixture, separating the lymphocyte cytoplasm content by centrifugation, thereby concentrating the lymphocyte cytoplasm content;
- and
- detecting the presence or absence of the a virus RNA or DNA in the lymphocyte cytoplasm content,
- wherein the presence of the virus RNA or DNA in the lymphocyte cytoplasm content, with the earlier non-detection of infection, indicates the preserved viability of viruses in said biological sample of said patient, and
- wherein the absence of the virus RNA or in the lymphocyte cytoplasm content indicates the loss of virus viability in said biological sample of said patient,
- whereby viruses with lymphotropism properties with a low virus concentration are detected in biological materials.
Dependencies are inferred from the claim text.