US 9,957,578 B2Granted

Genetic loci associated with resistance of soybean to cyst nematode and methods of use

AssigneePioneer Hi-Bred International, Inc.

InventorsBryce Reid Daines, David Hyten, Jr., Nichole Lynn Schneider, John Bryan Woodward

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Abstract

Various compositions and methods are provided for identifying and selecting plants with enhanced resistance to soybean cyst nematode (SCN). Further provided are transgenic plants, plant parts, and seed and methods of their use comprising a heterologous polynucleotide operably linked to a promoter active in the plant are provided, as are methods of making such plants and methods of use, wherein said heterologous polynucleotide comprises at least one, or any combination thereof, of Glyma18g2580, Glyma18g2590, Glyma18g2600, Glyma18g2610; and Glyma18g2570 or an active variant or fragment thereof. Expression of the heterologous polynucleotide enhances the resistance of the plant to cyst nematode.

Claims9

Dependent claims

A method of selecting a soybean plant or a soybean germplasm with resistance to a soybean cyst nematode, the method comprising:

(i) detecting, using quantitative PCR or other quantitative technique, in the genome of the soybean plant or in the genome of the soybean germplasm:
(a) a duplication of a region within the rhg1 locus;
(b) an increased copy number of at least SEQ ID NO:
5;
or
(c) a DNA junction formed at the breakpoint of a duplicated region within the rhg1 locus, wherein the DNA junction sequence is SEQ ID NO:
6;
(ii) selecting the soybean plant or the soybean germplasm having the duplication, increased copy number or DNA junction;
and
(iii) crossing the selected soybean plant with another soybean plant and selecting a progeny thereof having the duplication, increased copy number or DNA junction.

7 more elements

Dependencies are inferred from the claim text.

Facts

Application: 15/146,168Filed 4 May 2016 · division
Priority: 15 Jun 2012
Published: US 2016/0319377 A13 Nov 2016
Granted: 1 May 2018
Term ends: Not estimatedThe source records don’t support an estimate.

Classification11 codes

C12Q 1/6895
For detection or identification of organisms: for plants, fungi or algae
C07K 14/415
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from plants
C12N 15/8285
For biotic stress resistance, pathogen resistance, disease resistance: for nematode resistance
C12Q 2600/13
Oligonucleotides characterized by their use: plant traits

Family

Application61/660,387Us provisional application · filed 15 Jun 2012
Application61/671,937Us provisional application · filed 16 Jul 2012
Application61/740,526Us provisional application · filed 21 Dec 2012
Application13/779,957Parent of this division · filed 28 Feb 2013
US 2016/0319377 A1Pre-grant publication · published 3 Nov 2016
US 9,347,105 B2Parent of this division
US 2013/0340115 A1Published application
US 9,957,578 B2This patent

From the US filing history. Foreign family members aren’t listed.