Nucleic acid analysis device and nucleic acid analyzer

A nucleic acid analyzer for obtaining nucleotide sequence information of a nucleic acid sample, comprising:

• (i) a nucleic acid analysis device wherein microparticles having a probe molecule capable of capturing a nucleic acid to be detected are regularly immobilized by a hydrophobic interaction on hydrophobically treated bonding pads formed regularly separated from each other on a substrate,
• (ii) a means for supplying a nucleotide having a fluorescent dye, a nucleic acid polymerase, and a nucleic acid sample to the nucleic acid analysis device,
• (iii) a means for irradiating the nucleic acid analysis device with light, and
• (iv) a luminescence detector for measuring fluorescence of a fluorescent dye incorporated into a nucleic acid chain through a nucleic acid elongation reaction induced by the coexistence of the nucleotide, the nucleic acid polymerase, and the nucleic acid sample on the nucleic acid analysis device,
• wherein the microparticles are immobilized onto the bonding pads by the hydrophobic interaction via a linear molecule having an alkyl chain as a hydrophobic moiety, the alkyl chain including any one of alkylphosphate, alkyl sulfonic acid, and alkyl glycoside and having a length of 3 to 20 in terms of carbon number,
• wherein the microparticles are microspheres having an average diameter of 10 nm to 1 μm.