Microfluidic platform for multiplexed detection in single cells and methods thereof

A method for performing multiplexed, single-cell analysis in a microfluidic device, the method comprising:

• (i) loading a cell sample into a device, wherein the device comprises a plurality of holding chambers;
• (ii) holding a portion of the cell sample within each holding chamber, thereby providing a held cell;
• (iii) incubating the held cell with a first protein label in each holding chamber, wherein the first protein label is configured to detect a first target protein, thereby providing a labeled cell;
• (iv) incubating the labeled cell with a first nucleic acid label, wherein the first nucleic acid label is configured to detect a first target nucleic acid, thereby providing a multiplexed-labeled cell;
• (v) detaching the multiplexed-labeled cell, thereby providing a detached, intact cell;
• and
• (vi) performing an on-chip flow cytometry assay of the detached, intact cell, thereby performing multiplexed, single-cell analysis for the target protein and/or the target nucleic acid.