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US 9,957,550 B2Granted
Attenuators
AssigneeBioSpyder Technologies, Inc.
InventorsJoanne M. Yeakley, Bruce Seligmann, Joel McComb
Life of this patent
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Abstract
Methods for detecting nucleic acid sequences, where attenuator oligonucleotides are provided to reduce the number of detection products resulting from highly abundant sequences.
Claims37
A method for detecting a plurality of target nucleic acid sequences of interest in a sample, wherein each target sequence has an upstream region (UR) and a downstream region (DR), the method comprising:
- (a) contacting the sample with a pool of detector oligos comprising, for each target sequence,
- (1) a downstream detector oligo (DD) comprising a portion complementary to the downstream region of the target sequence (DR′), and
- (2) an upstream detector oligo (UD) comprising a portion complementary to the upstream region of the target sequence (UR′),
- wherein, for a predetermined set of potentially high abundance target sequences (HATs) in the sample, also contacting the sample with
- (3) an attenuator oligonucleotide that comprises a portion at least partially complementary to one of the downstream or upstream regions of a HAT, and further comprises a nonextendable or nonligatable blocking group, or a nonamplifiable segment;
- and
- (b) ligating a downstream detector oligo for a target sequence to a corresponding upstream detector oligo if both are hybridized to the downstream and upstream regions of a target sequence in the sample;
- thereby attenuating the number of HAT products to be detected.
5 more elements
Dependencies are inferred from the claim text.