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US 9,957,539 B2Granted
Method for using hexenol glycosyl transferase
AssigneeSuntory Holdings Limited
InventorEiichiro Ono
Life of this patent
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Abstract
A method for producing a hexenol glycoside using a hexenol glycosyltransferase. A transformant transformed with a gene encoding a hexenol glycosyltransferase and a method for preparing such a transformant.
Claims9
A method for producing a hexenol glycoside, comprising:
- contacting an isolated, purified or extracted protein with a UDP-sugar and a hexenol molecule to cause glycosylation of the hexenol molecule and to thereby produce a hexenol glycoside;
- wherein the isolated, purified or extracted protein has glycosylation activity on the hexenol molecule and is selected from the group consisting of:
- (a) a protein consisting of the amino acid sequence of SEQ ID NO:
- 2 or 4;
- (b) a protein consisting of the amino acid sequence of SEQ ID NO:
- 2 or 4, except that no more than 1 to 24 amino acids are deleted, substituted, inserted, and/or added;
- and
- (c) a protein comprising an amino acid sequence that has 95% or more sequence identity with the amino acid sequence of SEQ ID NO:
- 2 or 4.
6 more elements
A method for producing a hexenol glycoside, comprising:
- providing a cell comprising a polynucleotide encoding a protein, wherein said cell expresses said protein, and said cell is a microorganism or a transgenic plant cell, and
- contacting within or outside of the cell said protein with a UDP-sugar and a hexenol molecule to cause glycosylation of the hexenol molecule and to thereby produce a hexenol glycoside,
- wherein said polynucleotide comprises a nucleotide sequence encoding said protein and an expression control region that controls expression of said nucleotide sequence and wherein the nucleotide sequence encoding said protein and/or the expression control region is heterologous to said cell,
- wherein said protein has glycosylation activity on the hexenol molecule and is selected from the group consisting of:
- (a) a protein consisting of the amino acid sequence of SEQ ID NO:
- 2 or 4;
- (b) a protein consisting of the amino acid sequence of SEQ ID NO:
- 2 or 4, except that no more than 1 to 24 amino acids are deleted, substituted, inserted, and/or added;
- and
- (c) a protein comprising an amino acid sequence that has 95% or more sequence identity with the amino acid sequence of SEQ ID NO:
- 2 or 4.
8 more elements
Dependencies are inferred from the claim text.