US 9,957,536 B2Granted

Mutants of hydantoinase

AssigneeEvonik Technochemie GmbH

InventorsSteffen Osswald, Heiko Schuster, Jurgen Roos, Andreas Karau, Ulrich Schwaneberg, Ronny Martinez, Hemanshu Mundhada, Ursula Holter

Life of this patent

Estimating the term…

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Abstract

The present invention relates to a hydantoinase having an amino acid sequence selected from (i) or (ii), with (i) amino acid sequence selected from SEQ ID NO: 6-20 and SEQ ID NO: 73-119 (ii) amino acid sequence wherein in the amino acid sequence of SEQ ID NO: 6-20 and SEQ ID NO: 73-119, 1 to 75 amino acid residues have been substituted, deleted, inserted and/or added, and wherein further the catalytic activity of the hydantoinase is higher by a factor of at least 1.2 than the catalytic activity of the hydantoinase having amino acid sequence SEQ ID NO: 1, The present invention further relates to a process for preparing amino acids, wherein said hydantoinase is used.

A hydantoinase comprising an amino acid sequence selected from the group consisting of SEQ ID NO:

6, SEQ ID NO:
8, SEQ ID NO:
9, SEQ ID NO:
11, SEQ ID NO:
20, SEQ ID NO:
85, SEQ ID NO:
86, SEQ ID NO:
88, SEQ ID NO:
89, SEQ ID NO:
90, SEQ ID NO:
91, SEQ ID NO:
92, SEQ ID NO:
93, SEQ ID NO:
94, SEQ ID NO:
95, SEQ ID NO:
96, SEQ ID NO:
97, SEQ ID NO:
98, SEQ ID NO:
99, SEQ ID NO:
100, SEQ ID NO:
101, SEQ ID NO:
102, SEQ ID NO:
103, SEQ ID NO:
104, SEQ ID NO:
105, SEQ ID NO:
106, SEQ ID NO:
107, SEQ ID NO:
108, SEQ ID NO:
109, SEQ ID NO:
110, SEQ ID NO:
111, SEQ ID NO:
112, SEQ ID NO:
113, SEQ ID NO:
114, SEQ ID NO:
115, SEQ ID NO:
116, and SEQ ID NO:
117, or
an amino acid sequence selected from the group consisting of SEQ ID NO:
6, SEQ ID NO:
8, SEQ ID NO:
9, SEQ ID NO:
11, SEQ ID NO:
20, SEQ ID NO:
85, SEQ ID NO:
86, SEQ ID NO:
88, SEQ ID NO:
89, SEQ ID NO:
90, SEQ ID NO:
91, SEQ ID NO:
92, SEQ ID NO:
93, SEQ ID NO:
94, SEQ ID NO:
95, SEQ ID NO:
96, SEQ ID NO:
97, SEQ ID NO:
98, SEQ ID NO:
99, SEQ ID NO:
100, SEQ ID NO:
101, SEQ ID NO:
102, SEQ ID NO:
103, SEQ ID NO:
104, SEQ ID NO:
105, SEQ ID NO:
106, SEQ ID NO:
107, SEQ ID NO:
108, SEQ ID NO:
109, SEQ ID NO:
110, SEQ ID NO:
111, SEQ ID NO:
112, SEQ ID NO:
113, SEQ ID NO:
114, SEQ ID NO:
115, SEQ ID NO:
116, and SEQ ID NO:
117, wherein 1 to 50 amino acid residues have been substituted, deleted, inserted and/or added,
wherein the amino acid substitutions present in the amino acid sequence of SEQ ID NO:
6, SEQ ID NO:
8, SEQ ID NO:
9, SEQ ID NO:
11, SEQ ID NO:
20, SEQ ID NO:
85, SEQ ID NO:
86, SEQ ID NO:
88, SEQ ID NO:
89, SEQ ID NO:
90, SEQ ID NO:
91, SEQ ID NO:
92, SEQ ID NO:
93, SEQ ID NO:
94, SEQ ID NO:
95, SEQ ID NO:
96, SEQ ID NO:
97, SEQ ID NO:
98, SEQ ID NO:
99, SEQ ID NO:
100, SEQ ID NO:
101, SEQ ID NO:
102, SEQ ID NO:
103, SEQ ID NO:
104, SEQ ID NO:
105, SEQ ID NO:
106, SEQ ID NO:
107, SEQ ID NO:
108, SEQ ID NO:
109, SEQ ID NO:
110, SEQ ID NO:
111, SEQ ID NO:
112, SEQ ID NO:
113, SEQ ID NO:
114, SEQ ID NO:
115, SEQ ID NO:
116, and SEQ ID NO:
117, as compared to the amino acid sequence of SEQ ID NO:
1 are maintained,
wherein further the catalytic activity of the hydantoinase is higher by a factor of at least 1.2 than the catalytic activity of the hydantoinase having the amino acid sequence SEQ ID NO:
1, and wherein the catalytic activity of a hydantoinase is the catalytic activity of the hydantoinase with respect to transformation of a 5-substituted D-hydantoin compound to the corresponding D-carbamoylamino acid.

113 more elements

Facts

Application

14/357,028Filed 16 Nov 2012

Priority

EP 1118939516 Nov 2011

Published

US 2015/0056667 A126 Feb 2015

Granted

1 May 2018

Term ends

Not estimatedThe source records don’t support an estimate.

Classification8 codes

C12P 13/227
Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine: tryptophan
C12N 9/86
Acting on carbon to nitrogen bonds other than peptide bonds: acting on amide bonds in cyclic amides, e.g. penicillinase
C12P 13/04
Preparation of nitrogen-containing organic compounds: alpha- or beta- amino acids
C12P 41/009
By reactions involving C-N bonds, e.g. nitriles, amides, hydantoins, carbamates, lactames, transamination reactions, or keto group formation…

Abstract

Claims11