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US 9,957,529 B2Granted
Recombinant microorganism with improved butanol production ability and method for producing butanol by using the same
AssigneeGs Caltex Corporation
InventorsSang-Hyun Lee, Moon-Ho Eom
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Abstract
The present invention relates to a recombinant microorganism with improved butanol production ability which has an acetyl-CoA synthesis pathway and a butyryl-CoA synthesis pathway, wherein a pathway converting acetyl-CoA to acetate is inhibited and a pathway converting acetyl-CoA to butyryl-CoA is promoted. In addition, the present invention relates to a method for producing butanol using the recombinant microorganism.
Claims16
A recombinant microorganism with improved butanol production ability and decreased ethanol production ability in comparison to a wild type microorganism,
- wherein the microorganism is Clostridium acetobutylicum,
- wherein a pathway converting acetyl-CoA to acetate is inhibited, a pathway converting acetyl-CoA to butyryl-CoA is promoted, and a pathway converting butyryl-CoA to butyrate is inhibited,
- wherein the pathway converting acetyl-CoA to acetate is a pathway in which acetyl-CoA is converted to acetate by acetate kinase or phosphotransacetylase,
- wherein the pathway converting acetyl-CoA to butyryl-CoA is a pathway in which acetyl-CoA is converted to butyryl-CoA by thiolase or hbd-crt-bcd operon, and
- wherein the pathway converting butyryl-CoA to butyrate is the pathway in which butyryl-CoA is converted to butyrate by butyrate kinase.
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Dependencies are inferred from the claim text.