Recombinant protein expression using a hybrid CHEF1 promoter
AssigneeCmc Icos Biologics, Inc.
InventorHoward R. Clarke
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Abstract
The invention provides expression vectors and host cells for high-level expression of recombinant proteins. The expression vectors comprise Chinese hamster ovary elongation factor 1-α (CHEF1) transcriptional regulatory DNA elements and a cytomegalovirus (CMV) promoter and/or a human adenovirus tripartite leader (AdTPL) sequence. The invention achieves increased protein expression and better productivity of host cells compared to previously described expression systems.
Claims20
An expression vector comprising Chinese Hamster Elongation Factor-1α (CHEF1) transcriptional regulatory DNA and a cytomegalovirus (CMV) promoter and/or an adenovirus tripartite leader (AdTPL) sequence, wherein the expression vector comprises the polynucleotide set forth in SEQ ID NO:
- 6 or 7 or a polynucleotide at least 90% identical to the polynucleotide set forth in SEQ ID NO:
- 6 or 7.
A method of increasing protein expression, comprising transfecting a host cell with an expression vector comprising Chinese Hamster Elongation Factor-1a (CHEF1) transcriptional regulatory DNA, a cytomegalovirus (CMV) promoter and/or an adenovirus tripartite leader (AdTPL) sequence, and a polynucleotide encoding a protein of interest to increase the specific productivity of the protein of interest by the host cell by at least two-fold compared to an expression vector comprising a CHEF1 promoter without a CMV promoter and without an AdTPL sequence.
Dependencies are inferred from the claim text.