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US 9,957,511 B2Granted
Functionalization of endogenous bacteria
AssigneesMassachusetts Institute of Technology, Trustees of Boston University
InventorsTimothy Kuan-Ta Lu, Robert James Citorik, James Collins, Russell-John Krom
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Abstract
Various aspects and embodiments of the present disclosure are directed to methods and compositions for functionalizing endogenous bacteria in vivo. The methods include delivering to endogenous bacterial cells a recombinant bacteriophage or phagemid that is engineered to contain at least one genetic circuit.
Claims8
A method of functionalizing endogenous Escherichia coli (E. coli) in a subject, the method comprising delivering to a subject having an inflammatory bowel disease, non-lytic recombinant Inoviridae M13 coliphage that
- are not comprised within a bacterial cell during delivery,
- infect viable nonpathogenic endogenous E. coli present in the subject, and
- are engineered to contain at least one nucleic acid comprising an inducible promoter operably linked to a nucleotide sequence that encodes an interleukin (IL),
- to produce viable nonpathogenic functionalized E. coli in the subject.
1 more element
Dependencies are inferred from the claim text.