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US 9,957,509 B2Granted
Synthetic gene clusters
AssigneeThe Regents of the University of California
InventorsEthan Mirsky, Karsten Temme, Christopher A. Voigt, Dehua Zhao
Life of this patent
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Abstract
Methods for making synthetic gene clusters are described.
Claims16
A bacterial nitrogen reduction expression system comprising nucleic acids encoding:
- at least one operon comprising a plurality of coding sequences for a set of polypeptides encoded by genes collectively associated with nitrogen fixation within a cell, wherein at least one of the plurality of coding sequences comprises at least one non-native codon;
- a heterologous promoter region that directs expression of the at least one operon;
- and
- a heterologous transcriptional controller coding sequence that encodes a protein that directs expression of the at least one operon of the expression system, wherein the protein binds directly or indirectly to the heterologous promoter region.
1 more element
A bacterial nitrogen reduction expression system comprising nucleic acids encoding:
- at least one operon comprising a plurality of coding sequences for a set of polypeptides encoded by genes collectively associated with nitrogen fixation within a cell, wherein at least one of the plurality of coding sequences comprises at least one non-native codon;
- a heterologous promoter region that directs expression of the at least one operon, wherein the heterologous promoter region is from the same species as the genes collectively associated with nitrogen fixation;
- and
- a transcriptional controller coding sequence that encodes a protein that directs expression of the at least one operon of the expression system, wherein the protein binds directly or indirectly to the heterologous promoter region, and wherein the transcriptional controller does not regulate the genes collectively associated with nitrogen fixation under native regulation.
1 more element
A bacterial nitrogen reduction expression system comprising nucleic acids encoding:
- at least one operon comprising a plurality of coding sequences for a set of polypeptides encoded by genes collectively associated with nitrogen fixation within a cell, wherein at least one of the plurality of regulatory coding sequences has been synonymously mutated to remove internal regulation;
- a genetically engineered promoter region that directs expression of the at least one operon;
- and
- a transcriptional controller coding sequence that encodes a protein that directs expression of the at least one operon of the expression system, wherein the protein binds directly or indirectly to the heterologous promoter region, and wherein the transcriptional controller does not regulate the genes collectively associated with nitrogen fixation under native regulation.
1 more element
Dependencies are inferred from the claim text.