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US 9,957,493 B2Granted
Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
AssigneesNovozymes, Inc., Novozymes A/S
InventorsElena Vlasenko, Brett McBrayer, Mary Stringer, Kirk Schnorr
Life of this patent
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Abstract
The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Claims36
A nucleic acid construct comprising a polynucleotide encoding a polypeptide having cellobiohydrolase activity, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct the production of the polypeptide, and wherein the polypeptide having cellobiohydrolase activity is selected from the group consisting of:
- (a) a polypeptide comprising an amino acid sequence having at least 95% identity to the mature polypeptide of SEQ ID NO:
- 2;
- (b) a polypeptide encoded by a polynucleotide that hybridizes under very high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO:
- 1, or the genomic DNA sequence of the mature polypeptide coding sequence of SEQ ID NO:
- 1, wherein the very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide for 12 to 24 hours, and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 70° C.;
- and
- (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 95% identity to the mature polypeptide coding sequence of SEQ ID NO:
- 1.
5 more elements
A method for degrading or converting a cellulosic material, comprising:
- treating the cellulosic material with an enzyme composition comprising a polypeptide having cellobiohydrolase activity, wherein the polypeptide having cellobiohydrolase activity is selected from the group consisting of:
- (a) a polypeptide comprising an amino acid sequence having at least 95% identity to the mature polypeptide of SEQ ID NO:
- 2;
- (b) a polypeptide encoded by a polynucleotide that hybridizes under very high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO:
- 1, or the genomic DNA sequence of the mature polypeptide coding sequence of SEQ ID NO:
- 1, wherein the very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide for 12 to 24 hours, and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 70° C.;
- and
- (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 95% % identity to the mature polypeptide coding sequence of SEQ ID NO:
- 1.
6 more elements
A method for producing a fermentation product, comprising:
- (a) saccharifying a cellulosic material with an enzyme composition comprising a polypeptide having cellobiohydrolase activity, wherein the polypeptide having cellobiohydrolase activity is selected from the group consisting of:
- (i) a polypeptide comprising an amino acid sequence having at least 95% identity to the mature polypeptide of SEQ ID NO:
- 2;
- (ii) a polypeptide encoded by a polynucleotide that hybridizes under very high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO:
- 1, or the genomic DNA sequence of the mature polypeptide coding sequence of SEQ ID NO:
- 1, wherein the very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide for 12 to 24 hours, and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 70° C.;
- and
- (iii) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 95% identity to the mature polypeptide coding sequence of SEQ ID NO:
- 1;
- (b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product;
- and
- (c) recovering the fermentation product from the fermentation.
9 more elements
A method of fermenting a cellulosic material, comprising:
- fermenting the cellulosic material with one or more fermenting microorganisms, wherein the cellulosic material is saccharified with an enzyme composition comprising a polypeptide having cellobiohydrolase activity, wherein the polypeptide having cellobiohydrolase activity is selected from the group consisting of:
- (a) a polypeptide comprising an amino acid sequence having at least 95% identity to the mature polypeptide of SEQ ID NO:
- 2;
- (b) a polypeptide encoded by a polynucleotide that hybridizes under very high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO:
- 1, or the genomic DNA sequence of the mature polypeptide coding sequence of SEQ ID NO:
- 1, wherein the very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide for 12 to 24 hours, and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 70° C.;
- and
- (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 95% identity to the mature polypeptide coding sequence of SEQ ID NO:
- 1.
6 more elements
Dependencies are inferred from the claim text.