Pharmaceutical compositions and dermatogic compositions synthesized from catalytic domains producing highly α1,2 branched dextrans
AssigneeInsitut National Des Sciences Appliquees (Insa)
InventorsSophie Anne Michèle Bozonnet, Magali Martine Claude Remaud-Simeon, René-Marc Lucien Willemot, Pierre Emmanuel Frédéric Monsan
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Abstract
The invention relates to an isolated polypeptide with an glycosyl transferase enzymatic activity for producing dextrans with .alpha.(1.fwdarw.2) sidechains, comprising at least one region for bonding to glucan and a catalytically active region situated beyond the region bonding to glucan. The invention further relates to polynucleotides coding for said enzymes and vectors containing the same.
Claims12
A pharmaceutical or dermatologic composition comprising an isolated polypeptide having an enzymatic glycosyltransferase activity capable of forming dextrans having α(1→2) linkages from saccharose, a-D-fluoroglucose, paranitrophenyl-α-D glucopyranoside, α-D-glucopyranoside-α-D sorbofuranoside or 4-O-α-D galactopyranosylsucrose, wherein said isolated polypeptide has at least one glucan binding domain and a catalytic activity domain of SEQ ID No:1 located downstream of the glucan binding domain;
- and
- a suitable carrier.
Dependencies are inferred from the claim text.