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US 9,957,487 B2Granted
Mutated genes for the catalytic protein of Oplophorus luciferase and use thereof
AssigneeJnc Corporation
InventorsSatoshi Inouye, Junichi Sato
Life of this patent
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Abstract
Luciferases which are different from those known heretofore have been desired. A luciferase mutant comprising an amino acid sequence in which at least one amino acid selected from the group consisting of valine at the position of 44, alanine at the position of 54 and tyrosine at the position of 138 is substituted with other amino acid(s) in the amino acid sequence of SEQ ID NO: 2.
Claims8
A luciferase mutant defined in (a) or (b) below:
- (a) a luciferase mutant comprising the amino acid sequence of SEQ ID NO:
- 2 substituted at the positions consisting of the valine at position of 44, the alanine at position 54, and the tyrosine at position 138, wherein the luciferase mutant has luciferase activity;
- and
- (b) a luciferase mutant comprising the amino acid sequence of SEQ ID NO:
- 2 substituted at the positions consisting of:
- (1) the valine at position of 44, the alanine at position 54, and the tyrosine at position 138;
- and
- (2) one to sixteen positions, none of which includes any one of the positions 4, 11, 18, 27, 33, 43, 68, 72, 75, 90, 115, 124, and 166, wherein the luciferase mutant has luciferase activity.
5 more elements
Dependencies are inferred from the claim text.