Attenuation of human respiratory syncytial virus by genome scale codon-pair deoptimization
AssigneesThe United States of America, as represented by the Secretary, Dept. of Health and Human Services, The Research Foundation for The State University of New York
InventorsPeter L. Collins, Cyril Le Nouën, Linda G. Brock, Ursula J. Buchholz, Joshua Marc DiNapoli, Steffen Mueller, Eckard Wimmer
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Abstract
Described herein are RSV polynucleotide sequences that make use of multiple codons that are containing silent nucleotide substitutions engineered in multiple locations in the genome, wherein the substitutions introduce a numerous synonymous codons into the genome. Due to the large number of defects involved, the attenuated viruses disclosed herein provide a means of producing attenuated, live vaccines against RSV.
Claims12
A recombinant polynucleotide encoding a respiratory syncytial virus (RSV), wherein the nucleotide sequence encoding the RSV L protein comprises a sequence that is codon pair deoptimized from the corresponding sequence of a parent virus and the codon-pair deoptimization results in the nucleotide sequence encoding the RSV L protein being from about 70% to about 90% identical to the corresponding sequence of SEQ ID NO:1.
Dependencies are inferred from the claim text.