Methods for promoting cell reprogramming
AssigneeSanford-Burnham Medical Research Institute
InventorTariq M. Rana
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Abstract
The present invention is based on the seminal discovery that several kinases play important roles in barrier pathways in somatic cell reprogramming. The present invention provides that modulating expression or activity of these kinases can significantly promote or enhance cell reprogramming efficiency. Key kinases are identified and key regulation networks involving such kinases are also identified that may be advantageously targeted to significantly increase reprogramming efficiency as well as direct differentiation of induced pluripotent stem (iPS) cells.
Claims9
An in vitro method of generating an induced pluripotent stem (iPS) cell comprising:
- a) introducing into a human or mouse fibroblast one or more retroviral vectors encoding nuclear reprogramming factors OCT4, SOX2, KLF4 and c-MYC and b) introducing into the human or mouse fibroblast a nucleic acid that inhibits expression or activity of Aurora A kinase (AURKA) within the human or mouse fibroblast, and c) culturing the resulting human or mouse fibroblast in a cell media that supports growth of human embryonic stem (hES) cells or mouse embryonic stem (mES) cells, thereby generating an iPS cell.
Dependencies are inferred from the claim text.