Multiloop engineered heart muscle tissue
OwnerWolfram-Hubertus Zimmermann, Thomas Eschenhageninventors
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Abstract
The invention is directed to a method for the preparation of a multiring engineered heart tissue construct suitable for use in cardiac tissue augmentation and/or replacement therapy. The invention further refers to multiring EHT constructs which comprise at least two force-generating engineered heart tissue rings fused with each other and a device for preparing the same. Finally, the invention relates to force-generating engineered heart tissue rings derived from human cells and their use in drug screening and target validation assays.
Claims10
A method for ex vivo preparation of a multiring engineered heart tissue construct suitable for use in cardiac tissue-augmentation and/or -replacement therapy, comprising the steps of:
- (a) providing force-generating engineered heart tissue rings;
- (b) placing in position at least two of the force-generating engineered heart tissue rings so that each force-generating engineered heart tissue ring has one or more contact points to an adjacent force-generating engineered heart tissue ring;
- and
- (c) culturing the force-generating engineered heart tissue rings under conditions sufficient to fuse at least two of the force-generating engineered heart tissue rings at the one or more contact points to form the multiring heart tissue construct;
- wherein an amount of tensile stress sufficient to synchronize contractions of the rings is applied to at least two of the rings prior to and/or during step (c).
Dependencies are inferred from the claim text.