Cardiomyocyte production
AssigneeCellular Dynamics International, Inc.
InventorsCasey Stankewicz, Matt Riley, Nathaniel Beardsley, Wen Bo Wang, Peter Fuhrken, Steven Kattman
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Abstract
Methods and composition for the production of cardiomyocytes from differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including differentiating pluripotent stem cells in a large volume of suspension culture in the presence of ROCK inhibitors are described. In further aspects, methods for differentiation of stem cells into cardiomyocytes that overcome variability between different stem cell clones and different batch of culture medium are provided.
Claims14
An isolated cell population of about 106 to 1010 cells comprising human cardiomyocytes, wherein the cardiomyocytes are clonal, having been derived from a single clonal population of human iPS cells by differentiating the iPS cells into cardiomyocytes in vitro in the presence of one or more cytokines that promote cardiomyogenesis, and wherein at least 90% of the cells of the population are said human cardiomyocytes, wherein the cardiomyocytes comprise exogenous nucleic acid, but do not comprise an exogenous gene encoding a cardiac transcription factor.
Dependencies are inferred from the claim text.