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US 9,957,479 B2Granted
Mammalian haploid embryonic stem cells
OwnerAnton Wutz, Martin Leebinventors
Life of this patent
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Abstract
The invention relates to mammalian haploid embryonic stem cells and methods for the production of such stem cells. The inventions also relates to a cell culture and a cell line of mammalian haploid embryonic stem cells.
Claims6
A mammalian haploid embryonic stem cell line, wherein the stem cell line is pluripotent, proliferates, and maintains a haploid karyotype during proliferation in culture for a minimum of 45 days, wherein the cell line is cultured in conditions selected from:
- a) a N2B27 chemically defined medium comprising LIF and one or more 2i inhibitor;
- b) a N2B27 chemically defined medium comprising 15% KnockOut Serum Replacement supplemented with Glutamin, betamercaptoethanol, penicillin-streptomycin, non-essential amino acids, and 500 units per milliliter recombinant mouse LIF;
- c) a N2B27 chemically defined medium comprising 15% fetal calf serum supplemented with Glutamin, betamercaptoethanol, penicillin-streptomycin, non-essential amino acids, and 500 units per milliliter recombinant mouse LIF;
- d) high glucose DMEM medium or KnockOut DMEM medium comprising 15% fetal calf serum supplemented with Glutamin, betamercaptoethanol, penicillin-streptomycin, non-essential amino acids and 500 units per milliliter recombinant mouse LIF;
- or
- e) high glucose DMEM medium or KnockOut DMEM medium comprising 15% KnockOut Serum Replacement supplemented with Glutamin, betamercaptoethanol, penicillin-streptomycin, non-essential amino acids, and 500 units per milliliter recombinant mouse LIF.
3 more elements
Dependencies are inferred from the claim text.