Method for enzymatic treatment of tissue products
AssigneeLifeCell Corporation
InventorsYi Chen, Hui Xu, Li Ting Huang, Wenquan Sun, Hua Wan
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Abstract
Methods for treating tissue matrices and tissue matrices produced according to the methods are provided. The methods can include treating a tissue matrix with a proteolytic enzyme to produce a desired pliability of the tissue matrix and/or to control the immunogenicity of the tissue matrix. The methods can also comprise performing an assay to determine if contacting the at least one collagen-containing tissue matrix with a proteolytic enzyme has altered the at least one collagen-containing tissue matrix to reduce a human immune response to the tissue matrix.
Claims13
A method for treating a tissue matrix, comprising:
- selecting at least one collagen-containing tissue matrix;
- contacting the at least one collagen-containing tissue matrix with a first solution comprising a proteolytic enzyme to create an enzyme-treated tissue matrix;
- and
- treating the enzyme-treated tissue matrix with a second solution to remove at least some of the cells and cellular components from the enzyme-treated tissue matrix,
- wherein the enzyme comprises alcalase and is contacted with the tissue matrix under conditions sufficient to produce a desired level of pliability in the tissue matrix and to produce a desired increase in porosity of the tissue matrix and reduce an immunogenicity of the tissue matrix when implanted in a human recipient.
Dependencies are inferred from the claim text.