Enzymatic hydrolysis of acylated steryl glycosides and method for treating biofuel
AssigneeETH Zurich
InventorsLinda Munger, Laura Nystrom
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Abstract
The present invention relates to the use of at least one glycosidase of the class EC 3.2.1.21, EC 3.2.1.4, EC 3.2.1.7, and/or EC 3.2.1.80 for hydrolyzing at least one glycosiding bond in at least one substrate selected from the group of acylated steryl glycosides of the general formula (I): R1—CO—O—[CmHnOx]y—O—R2 wherein R1 is a saturated or non-saturated C1-C30 alkyl, R2 is a sterol moiety, m=5-7, n=9-14 and x=5-7, y=1-5. The present invention relates further to a method of for hydrolysing steryl gylcosides using said enzymes in biofuel, oil and/or fat.
Claims17
A method for hydrolyzing at least one glycoside bond in at least one substrate selected from the group of acylated steryl glycosides of the general formula I
- R1—CO—O—[CmHnOx]y—O—R2
- wherein
- R1is a saturated or non-saturated C1-C30 alkyl,
- R2is a sterol moiety,
- m=5-7, n=9-14 and x=5-7,
- y =1-5
- using at least one glycosidase of the class EC 3.2.1.7 and/or EC 3.2.1.80.
A method for hydrolyzing at least one acylated steryl glycoside and/or at least one steryl glycoside in biofuel, oil and/or fat by mixing at least one glycosidase of the class EC 3.2.1.7 and/or EC 3.2.1.80 with the biofuel, oil or fat.
Dependencies are inferred from the claim text.