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US 9,957,328 B2Granted
Bacterial host strain expressing recombinant DSBC
AssigneeUCB Pharma S.A.
InventorsMark Ellis, David Paul Humphreys
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Abstract
The present invention provides a recombinant gram-negative bacterial cell comprising an expression vector comprising a recombinant polynucleotide encoding DsbC and one or more polynucleotides encoding an antibody or an antigen-binding fragment thereof specifically binding to CD154.
Claims27
A recombinant gram-negative bacterial cell comprising the following genetic modifications:
- (a) an expression vector comprising a recombinant polynucleotide encoding a DsbC protein;
- (b) a polynucleotide encoding a periplasmic Pre protease (Tsp), said polynucleotide comprising nucleotides 7 to 2048 of SEQ ID NO:
- 25 or comprising SEQ ID NO:
- 28;
- (c) one or more polynucleotides encoding an antibody or an antigen binding fragment thereof specifically binding to CD154;
- and
- (d) optionally, a polynucleotide encoding a mutant extragenic suppressor (spr) protein,
- wherein the cell is isogenic to a wild-type bacterial cell except for the genetic modifications (a) to (c) and when present, (d).
5 more elements
Dependencies are inferred from the claim text.