Method for detecting bilirubin using a fluorescent protein
AssigneeRiken
InventorsAtsushi Miyawaki, Akiko Kumagai
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Abstract
In order to provide a novel fluorescent protein and use thereof, the polypeptide according to the present invention has fluorescent properties in the presence of bilirubin and includes (1) the amino acid sequence of SEQ ID NO: 1, (2) an amino acid sequence having, for example, substitution of 1 to 21 amine acids in the amino acid sequence of SEQ ID NO: 1, (3) an amino acid sequence having 85% or more sequence identity to the amino acid sequence of SEQ ID NO: 1, or (4) the amino acid sequence encoded by a polynucleotide that hybridizes with a polynucleotide consisting of a sequence complementary to the polynucleotide encoding the polypeptide according to the amino acid sequence in (1) under a stringent condition.
Claims10
A method for detecting bilirubin in a subject, the method comprising:
- contacting a sample from the subject with a polypeptide, wherein the polypeptide has fluorescent activity upon binding to bilirubin;
- and
- detecting fluorescence emitted by the polypeptide after the contacting step,
- wherein the presence of fluorescence emission detects bilirubin in the subject, and
- wherein the polypeptide is selected from the group consisting of;
- (1) a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 1;
- (2) a polypeptide comprising an amino acid sequence that has at least 95% sequence identity to the amino acid sequence of SEQ ID NO:
- 1;
- (3) a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 1, except for an amino substitution selected from the group consisting of Al2E, S80N, R82E, K84E, and combinations thereof in the amino acid sequence of SEQ ID NO:
- 1;
- and
- (4) a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 5 or SEQ ID NO:
- 29.
Dependencies are inferred from the claim text.